Assessing the quality of drinking water from selected water sources in Mbarara city, South-western Uganda.

This study assessed the physical, chemical, and microbiological quality with emphasis on risk score, source apportionment, geochemistry, feacal coliforms and water quality index of drinking water from selected water sources. A cross-sectional study was conducted in six villages in Mbarara city, south-western Uganda. Each selected source was inspected using a WHO-adopted sanitary inspection questionnaire. Each source's risk score was calculated. Thirty-seven samples were taken from one borehole, nine open dug wells, four rain harvest tanks, and twenty-three taps. The values for apparent color and phosphate were higher than the permissible level as set by the World Health Organization and Ugandan standards (US EAS 12). The isolated organisms were Klebsiella spp. (8.11%), Citrobacter divergens (62.16%), Citrobacter fluendii (2.7%), E. coli (35.14%), Enterobacter aerogenes (8.11%), Enterobacter agglomerus (5.4%), Proteus spp. (2.7%), Enterobacter cloacae (13.5%), and Proteus mirabilis (2.7%). Twelve water sources (32.4%) had water that was unfit for human consumption that was unfit for human consumption (Grade E), Five sources (13.5%) had water that had a very poor index (Grade D), nine (24.3%) had water of poor index (Grade C), eight (21.6%) had water of good water index (Grade B), and only three (8.1%) had water of excellent water quality index (Grade A). The piper trilinear revealed that the dominant water type of the area were Mgso4 and Caso4 type. Gibbs plot represents precipitation dominance. PCA for source apportionment showed that well, tap and borehole water account for the highest variations in the quality of drinking water. These results suggest that drinking water from sources in Mbarara city is not suitable for direct human consumption without treatment. We recommend necessary improvements in water treatment, distribution, and maintenance of all the available water sources in Mbarara City, South Western Uganda.

Ethnobotanical study of medicinal plants utilized in the management of candidiasis in Northern Uganda.

BACKGROUND: The emergence of resistant Candida species to antifungal drugs has led to resurgence in herbal usage globally. However, little is known about anti-candida plants. This study explored ethnomedicinal plants as treatment option for candidiasis in Pader, Northern Uganda. METHODS: A cross-sectional survey of potential anti-candida plants was conducted using questionnaires, focus group discussions and field observations in March 2022. Sixty-three respondents were selected by snowball technique. The frequencies of respondents/responses were analyzed, associations of respondents' socio-demographics with indigenous knowledge of herbal usage established by Chi-square (χ2) test using SPSS 27. Informant Consensus Factor was computed to establish level of agreement on herbal usage, and thematic analysis done for focus group discussions. RESULTS: Candidiasis is still common and troublesome in Pader. All herbalist had equal chances of receiving and treating candidiasis patients irrespective of herbalist's gender, age, education level, occupation, marital status and religion (p > 0.05). About 39.7% of herbalists received candidiasis patients weekly (p < 0.01). All herbalists had knowledge on candidiasis. Death (56.8%) and discomfort (36.8%) were the major health risks of oropharyngeal candidiasis (OPC) and vulvovaginal candidiasis (VVC), respectively. A total of 32 potential anti-candida plant species in 18 families were identified. Families of Fabaceae (9 species) and Asteraceae (5 species) had most plant species. Trees (50.0%) and herbs (43.8%) were the dominant life forms. The commonest plants by frequency of mention were: Momordica foetida (26), Sansevieria dawei (20), Khaya anthotheca (15), Piliostigma thonningii (10), Clerodendrum umbellatum (7), Hallea rubrostipulata (5) and unidentified plant, 'Agaba/daa layata' in Acholi dialect (5). Plant parts mainly used were roots (56.3%) and stem barks (15.6%) harvested majorly by cutting (46.9%) and uprooting (12.5%). Most respondents (females, 95%) preferred herbal to western medication (p < 0.01) due to its perceived effectiveness. There was high consensus among herbalists on herbal remedies for OPC and VVC (FIC = 0.9). CONCLUSIONS:  Pader communities have diverse indigenous knowledge on candidiasis and prefer herbal medicines to orthodox treatment for candidiasis. However, the herbalists use unsustainable harvesting techniques like uprooting whole plants and cutting main roots. Hence, the need to document such indigenous knowledge before being lost for community usage and scientific validation.

Pumpkin and watermelon production constraints and management practices in Uganda.

Background: Watermelons and pumpkins are cultivated in Uganda for their leaves, fruits, and seeds, thereby contributing to food, nutrition and income security. However, there is limited research and information on constraints affecting their production. This study assessed the current production constraints for watermelons and pumpkins, management practices, sources of production inputs to guide research and decision making in production of these crops. Methodology: Watermelon and pumpkin fields totalling 105 located in 28 districts from nine sub-regions of Uganda were surveyed. Purposive sampling was conducted based on the importance and availability of watermelon and pumpkin fields in the sub-regions using a questionnaire administered to farmers on different practices, management strategies, and current production constraints. Data were analysed to determine the relationship between the source of seed, sale of their produce, constraints, and control measures of biotic constraints in the different sub-regions. Results: Pumpkins and watermelons were grown by 85.7% and 14.3% of respondent farmers, respectively. The constraints as ranked by the farmers were pests, diseases, drought, high transport and labour costs. Bacterial wilt, downy mildews, anthracnose powdery mildews and virus diseases in this order were the most common and important disease constraints.The whitefly (Bemisia tabaci, Gennadius), order hemiptera family aleyrodidae, aphids (Myzus Persicae, Sulzer), order hemiptera family aphidadae, melon fly (Bactrocera cucurbitae, Coquillett), order diptera family tephritidae and cutworm (Agrotis ipsilon, Hufnagel), order lepidoptera family noctuidae, were reported as the most limiting pests of watermelon and pumpkin production. Mixing of several agrochemicals was observed in watermelon fields coupled with gross lack of knowledge of proper usage or purpose of these chemicals may result in pesticide resistance, health and environmental hazards. Conclusion: Pests, diseases, and drought constitute the main constraints affecting watermelon and pumpkin cultivation in Uganda. Whereas weeding using hand hoes is the most common method of weed control, application of ash was the main strategy for pest management in pumpkin, while in watermelons, pheromone traps and pesticides were frequently used.